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課程名稱 |
質譜技術應用
Application of Mass Spectrometry |
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開課學期 |
106-2 |
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授課對象 |
公共衛生學院 食品安全與健康研究所 |
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授課教師 |
陳鑫昌 |
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課號 |
EH7035 |
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課程識別碼 |
844 M1390 |
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班次 |
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學分 |
2.0 |
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全/半年 |
半年 |
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必/選修 |
必修 |
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上課時間 |
星期一3,4(10:20~12:10) |
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上課地點 |
公衛211 |
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備註 |
限碩士班以上
總人數上限：40人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1062EH7035_IFSHMS |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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課程概述 |
本課程於兼顧基礎理論與實際應用的前提下，介紹常用的游離源、質量分析器及掃描模式。亦會介紹如何利用NIST及ChemSpider等資料庫搜尋進行未知物定性，及以非目標物檢測模式分析非正面表列之農藥、動物用藥及其他未知物分析。利用所學之質譜技術應用於食品中微量污染物及毒性化學物質的定性/定量，以及生物樣品(血液及尿液)中代謝物之分析。
1. This course focuses on frequently used ionization and detection modes, their suitable samples and analytes, the compatible interfaces such as gas, liquid, and ion chromatography, and library searching systems such as NIST and ChemSpider.
2. Non-target monitoring with mass spectrometry will also be included especially for pesticides and veterinary drugs.
3. The fundamental knowledge, which includes sampling and analysis, for the qualification and quantification of contaminants and toxic chemicals at ultra-race levels in various food matrix and biological samples will also be noted. |
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課程目標 |
學生於修課後能瞭解當今食品安全常用的技術原理與適合之應用層面，了解樣品與分析物該使用何種層析系統如氣相層析儀或液相層析儀搭配適當的游離源及質量分析器進行分析，並說明質譜分析品質保證之標準，期望學生能抓住相關文獻或演說中有關食品安全及質譜技術之重要論點。
1. Enabling the students to learn the principles of mass spectrometry, to evaluate the adaptability and the necessity of this technique to certain food safety studies
2. To understand the importance and knowledge of unbiased evaluation of distribution of contaminants in food.
3. Offering a problem-based approach to analytical quality assurance |
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課程要求 |
無
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預期每週課後學習時數 |
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Office Hours |
另約時間 |
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指定閱讀 |
Mass Spectrometry: Principles and Applications 3rd Ed., Edmond de Hoffmann and Vincent Stroobant, John Wiley & Sons Ltd, 2007 |
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參考書目 |
1.Mass Spectrometry: A Textbook, Gross, Jürgen H, Springer, 2017 (e-book available)
2.Ultra Performance Liquid Chromatography Mass Spectrometry: Evaluation and Applications in Food Analysis, Mu Naushad, Mohammad Rizwan Khan, Taylor & Francis Group, LLC, 2014 (e-book available)
3.Practical Gas Chromatography: A Comprehensive Reference, Katja Dettmer-Wilde and Werner Engewald, by Springer-Verlag Berlin Heidelberg, 2014 (e-book available)
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評量方式
(僅供參考) |
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No. |
項目 |
百分比 |
說明 |
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1. |
期中考 (Midterm exam) |
50% |
期中考紙筆測驗 |
2. |
小組報告 (Group projects of Special Topics) |
40% |
分組報告，選定指標性期刊之文章進行文獻回顧、報告、心得及建議。 |
3. |
書面報告 (Projects): |
10% |
小組報告之資料整理，整理成書面資料繳交。 |
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週次 |
日期 |
單元主題 |
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第1週 |
2/26 |
History and main domains of mass spectrometry
Resolution, Isotopic Composition, and Accurate Mass |
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第2週 |
3/05 |
Sample inlet, ion source, and mass analyzer |
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第3週 |
3/12 |
Ion sources: EI, CI, ICP, FAB, SIMS |
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第4週 |
3/19 |
Ion sources: MALDI, API (ESI, APCI, APPI) |
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第5週 |
3/26 |
Mass analyzers: B/E sector, TOF, IMS, FT and Orbitrap |
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第6週 |
4/02 |
溫書假_停課 |
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第7週 |
4/09 |
Mass analyzers: quadrupole, ion trap, Linear ion trap, QqLIT
Tandem mass spectrometry (MS/MS) and IRMS |
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第8週 |
4/16 |
Mass Analyzer (III), detector and Vacuum System |
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第9週 |
4/23 |
Midterm exam |
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第10週 |
4/30 |
Interpretation of mass spectra |
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第11週 |
5/07 |
Coupling to chromatography: GC-MS, GC-MS/MS, GCxGC-MS and application |
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第12週 |
5/14 |
Coupling to chromatography: LC-MS, LC-MS/MS, and application |
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第13週 |
5/21 |
Method validation in the quality assurance/quality control |
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第14週 |
5/28 |
Non-Target Screening for pesticide and veterinary drugs
分組報告1 (夏瑞敏、翁仕哲、陳婷甄、林尚廷、張景翔) |
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第15週 |
6/04 |
Special topics in application of food safety and food fraud (I) |
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第16週 |
6/11 |
Special topics in application of food safety and food fraud (II)
分組報告1 (何采築、蔡瑋珹、黃捷、陳怡儒、張書維)
分組報告2 (孫一誠、邱翊、詹文君、賴昆暉) |
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第17週 |
6/18 |
端午節(放假日) |
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第18週 |
6/25 |
Special topics in biological Analysis of chemicals/metabolites from food
分組報告1 (趙澤瑋、張慈庭、許瀞尤、陳品儒)
分組報告2 (蕭鵬、蘇偉綸、蕭佑亭、許小璿) |
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